Tips for Auto Nucleic Acid Extractor Use in PCR Laboratory
2021-08-28 09:56
Nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete sample nucleic acid extraction. It is widely used in various fields such as the Center for Disease Control, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiological testing, food safety testing, animal husbandry and molecular biology research.
Nucleic acid extractors are divided into two types. One is a large-scale automatic type, generally called an automatic liquid workstation, and the other is a small-scale automatic nucleic acid extractor, which uses packaged supporting reagents to automatically complete the extraction and purification process. Because of the high equipment cost and high operating cost, large-scale automatic liquid workstations are suitable for extracting thousands of specimens of the same type at a time, so there are fewer applications, while small-scale automatic nucleic acid extractors are more commonly used due to its low cost of equipment and operation as well as easy operation.
Precautions for use of automatic nucleic acid extractor:
1. Installation environment: It requires normal atmospheric pressure (altitude should be lower than 3000m), temperature 20-35°C, typical use temperature 25°C, relative humidity 10%-80%, unobstructed air flow 35°C or below.
2. It is not allowed to place the instrument near a heat source, such as an electric heater. At the same time, do not splash water or other liquids into it so as to prevent short circuits of electronic components.
3. Both the air inlet and outlet are located on the back of the instrument. At the same time, in order to prevent dust or fibers from gathering at the air inlet, make sure to keep the air duct unobstructed.
4. The nucleic acid extractor is at least 10cm away from other vertical surfaces.
5. Grounding: In order to avoid electric shock accidents, the input power cord of the instrument must be properly grounded.
6. Away from live circuits: Operators are not allowed to disassemble the instrument without authorization. Replacement of components or internal adjustments must be completed by qualified professional maintenance personnel. Do not replace components when the power is turned on.
The nucleic acid detection aims at the nucleic acid of the virus, and in the sample nucleic acid extraction, the nucleic acid extractor is an indispensable instrument. With the advancement of scientific research and technology, small automatic nucleic acid extractors have gradually become popular, and are no longer limited by cost and technology, and can be widely used in any laboratory.