Design: layout, HVAC design
Configuration: Hydropower, equipment
The current technical specifications and standards for the construction and management of PCR laboratories:
*PCR laboratory should meet the second level of biosafety requirements
*"For operations involving clinical samples, it should meet the requirements of biosafety secondary laboratory protective equipment, personal protection and operating specifications."
Basis of construction：
"Laboratory Biosafety Handbook" —World Health Organization
"Building Technical Code for Biosafety Laboratory"
"Code for Construction Quality Acceptance of Building Water Supply, Drainage and Heating Engineering"
"Code for Quality Acceptance of Building Decoration Engineering"
Technical specifications for the construction of biosafety grade laboratories:
According to the biological hazards of the objects handled by the laboratory and the protective measures taken, the biosafety laboratory classifies the drivers. Microbiological biosafety laboratories can use BSL-1, BSL-2, BSL-3, BS-4 to represent the corresponding level of laboratories. The biosafety laboratory is divided into the following:
Low individual hazards, low group hazards
Low harm to human body, animals and plants or the environment, without pathogenic factors for healthy adults, animals and plants
Medium individual hazard, limited group hazard
It is a moderately harmful or potentially dangerous pathogenic factor to human body, animals and plants or the environment. It will not cause serious harm to healthy adults, animals and plants, and has effective prevention and treatment measures.
High individual hazard, low group hazard
Highly harmful to the human body, animals and plants or the environment, causing serious or even fatal diseases through direct contact or aerosol, or pathogenic factors that are highly harmful to animals, plants and the environment.Usually by preventive and therapeutic measures
High individual hazard, high group hazard
Highly harmful to human body, animals, plants or environment, spread through aerosol or unknown transmission route, unknown, highly dangerous pathogenic factors. No preventive and therapeutic measures
Laboratory Biosafety General Requirements:
The doors of the main entrance of the laboratory and the rooms where the biological safety cabinets door are placed should be automatically closed.
The laboratory can use natural ventilation. If mechanical ventilation is used, cross infection should be avoided.
Eyewash devices should be equipped in the laboratory entry and work area.
Laboratory Biosafety Manual —WHO
Guidelines for the work of clinical gene amplification laboratory in medical institutions
The design of the clinical gene amplification test laboratory:
The regional design plan of the clinical gene amplification test laboratory. In principle, the clinical gene amplification laboratory should set up the following areas: reagent storage and preparation area, specimen preparation area, amplification area, and amplification product analysis area. These four areas must be completely independent of each other in physical space. Whether in space or in use, each area should always be in a completely divided state without direct air communication. Depending on the function of the instrument used, the areas can be combined appropriately. If using a real-time fluorescent PCR instrument, the amplification area and the amplification product analysis area can be combined; using an automated analyzer that integrates specimen processing, nucleic acid extraction and amplification detection, the specimen preparation area, amplification area, and amplification product analysis area Can be merged.