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PCR Laboratory

Brand:NANBEI
Model:PCR Laboratory
Application:Laboratory,School,Hospital...

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Product Details

PCR Laboratory

The PCR laboratory is mainly located in the laboratory and pathology department. It can be amplified and analyzed in vitro. To diagnose the prevalence of infectious diseases, cancer, AIDS, hepatitis A, hepatitis B, tuberculosis, syphilis, rubella and other patients. It is usually used for early diagnosis of diseases and various treatment stages. Through the DNA gene tracking system, can quickly grasp the virus content in the patient's body, and its accuracy is as high as nanometers.

1. Reagent preparation area

Function: It is mainly used for the preparation of storage reagents, the packaging of reagents and the preparation of the main reaction mixture. The reagents and materials used for specimen preparation should be transported directly to this area, and should not pass through other areas. Reagent raw materials need to be stored in this area, and the required storage reagents are prepared in this area.
Reagent preparation room: configuration of experimental reagents.
Reagent configuration steps: Weigh solid medicines with a balance or pipette to take liquid reagents (mix), add ultrapure water to dissolve, can configure electrophoretic solution, sodium acetophenate solution, reagent configuration, can be operated in an ultra-clean workbench.

Category	Selection Purpose
Balance	Used for accurate weighing of various reagents
Ultra-clean workbench	All operations involving bacteria must be done under the ultra-clean workbench.
Pipette	Accurately measure a specific volume of solution
Water purifier	Ultra pure water
Medicine refrigerator	Short-term storage
Reagent low temperature refrigerator	Optional 2-8℃、-20℃
Mixer	Need to mix before transferring the sample
Disinfection vehicle	Space environment disinfection
Sterilization pot	Sterilization and sterilization of laboratory items, reagents, culture media
PH meter	Configuration reagent is accurate measurement of PH value

2. Sample preparation area

Function: preservation of clinical specimens, nucleic acid (RNA, DNA) extraction, storage and DNA synthesis when added to amplification reaction tubes and RNA determination.
Sample preparation room: nucleic acid extraction and purification.
Extraction: Pipette the sample after mixing, add 0.5% SDS and protease to digest at 70°C (constant temperature water tank), add phenol and chloroform.

Category	Selection Purpose
Biological safety cabinet	To prevent the spread of harmful suspended particles and aerosols
Sample Refrigerator	Store samples and DNA in -20°C, -80°C
High-speed tabletop refrigerated centrifuge	It is used to collect microbial cells, cell debris and other sediments. Some samples require low temperature environment because they are not stable at normal temperature.
Water bath or metal bath	Used for temperature control of water bath during DNA hybridization
Pipette	Accurately measure a specific volume of solution
Tips	Matching pipettes
Ultraviolet lamp or disinfection vehicle	Space environment disinfection
Mixer	Need to mix before transferring the sample
Ultra-clean workbench	All operations involving bacteria must be done under the ultra-clean workbench.
Low temperature press	For shaking culture of E. coli and yeast
Magnetic stirrer	Accelerates the dissolution of solid content

3. Nucleic acid amplification area

Function: Mainly DNA amplification, prepared DNA template and synthesized DNA and main reaction mixture, prepared into reaction mixture, etc., can also be carried out in this area. In PCR measurement, the reaction tube is usually opened after amplification.

Category	Selection purpose
Gene Amplifier	Genetic testing DNA/RNA amplification
Fluorescence quantitative PCR instrument	Nucleic acid quantitative analysis
Nucleic acid extractor	Sample DNA/RNA extraction
Pipette	Accurately measure a specific volume of solution
UV lamp or disinfection vehicle	Space environment disinfection
Ultra-clean workbench	Operations involving bacteria must be completed under an ultra-clean workbench.
Water purifier S	For PCR, DNA sequencing requires ultrapure water

4. Product analysis area

Function: preservation of clinical specimens, nucleic acid (RNA, DNA) extraction, storage and DNA synthesis when added to amplification reaction tubes and RNA determination.
Sample preparation room: nucleic acid extraction and purification.
Extraction: Pipette and mix the sample, add 0.5% SDS and protease to digest at 70°C (constant temperature water tank), add benzene and chloroform.

Category	Selection purpose
Electrophoresis	Horizontal electrophoresis is used for nucleic acid DNA/RNA detection, vertical electrophoresis is used for protein detection, and transfer electrophoresis is used to transfer protein to the membrane for western detection.
Gel imaging system/UV detector	For nucleic acid agar electrophoresis and protein polyacrylamide observation and photographing; UV analyzer is used for nucleic acid agar electrophoresis gel observation after staining, can not be connected to a computer
Electric stove	Electrophoresis agar gel melted by heating

Zhengzhou NANBEI Instrument Equipment Co.,Ltd

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PCR Laboratory Setting Up Basis