Electrophoresis experiment is one of the main techniques in molecular biology research. The electrophoresis instrument consists of a host and an electrophoresis tank. The electrophoresis tank includes a vertical electrophoresis tank, a horizontal electrophoresis tank and an electrotransfer.
The power supply of electrophoresis cell is different from other power supplies. It requires constant voltage, constant current, and constant power. The following points should be noted when choosing it:
1. The range of constant voltage and constant current of the power supply:
Generally, it is determined according to the molecular weight of the nucleic acid or protein. As long as it is not a particularly large protein, the parameters of 0-300v (electrophoresis) and 0-400mA (electrotransfer) are ok.
2. The number of output ports
When purchasing, pay special attention to the number of power output ports. Most power supplies have 4 holes.
3. Step-by-step operation function
During electrophoresis, concentrated gel and separation gel are generally set. These two parts of gel require different voltages. Therefore, when choosing a power supply, in order to avoid constantly adjusting parameters, it is better to choose a power supply with step-by-step operation function.
When using the electrophoresis system, pay attention to cleanliness. After the experiment, pour out the electrophoresis solution in time, rinse it clean, dry it and keep it dry. After each experiment, the electrodes should be wiped and kept dry in time to prevent rust.
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